Anti-Human HLA-DP (MHC Class II, Clone B7/21) Monomorphic - PE

Antibody Details

Product Details

Reactivity Species

Human

Expression Host

HEK-293

Product Concentration

0.2 mg/ml

Formulation

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

RRID

AB_2892892

Applications and Recommended Usage?
Quality Tested by Leinco

FC

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Anti-Human HLA-DP (Clone B7/21) recognizes a monomorphic epitope on human HLA-DP1, -DP2, -DP3, -DP4, and -DP5. It does not cross-react with HLA-DR or HLA-DQ.

Antigen Distribution

HLA-DP is expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells.

Background

This Recombinant HLA-DP antibody, clone B7/21, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen - DP isotype (HLA-DP). MHC class II is constitutively expressed on human professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation¹. HLA-DP consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain² encoded by the HLA-DPA1 and HLA-DPB1 genes, respectively, located in the HLA complex of chromosome 6. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens³. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling⁴. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation⁵ and can transduce signals into T cells, enhancing T cell proliferation and activity⁶. High HLA-DP expression is associated with an increased risk of graft-versus-host disease⁷. Specific alleles of HLA-DP are associated with autoimmune diseases, including rheumatoid arthritis⁸.

Antigen Details

Protein

HLA-DP Monomorphic

PubMed

HLA-DP Monomorphic

Research Area

Immunology

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Innate Immunity

References & Citations

1. Holling TM, et al. (2004) Hum Immunol. 65(4):282-90.

2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25.

3. Wieczorek M, et al. (2017) Front Immunol. 8:292.

4. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921.

5. Barnaba V, et al. (1994) Eur J Immunol. 24(1):71-5.

6. Di Rosa F, et al. (1993) Hum Immunol. 38(4):251-60.

7. Petersdorf EW, et al. (2015) N Engl J Med. 373(7):599-609.

8. Raychaudhuri S, et al. (2012) Nat Genet. 44(3):291-6.

Technical Protocols