Anti-Human CD62L - Purified In vivo PLATINUM Functional Grade

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

< 0.5 EU/mg as determined by the LAL method

Purity

≥98% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2829786

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this DREG56 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this DREG56 antibody for use in western blotting is 1-10 μg/ml.

Other Applications Reported In Literature ?

IHC FF
IF
B

Additional Reported Applications For Relevant Conjugates ?

CODEX®

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone DREG56 recognizes an epitope on human CD62L.

Antigen Distribution

CD62L is expressed on most circulating leukocytes, including neutrophils, monocytes, T, B and NK cells, and human embryo trophoblasts.

Background

CD62L antibody, clone DREG56, recognizes CD62L, also known as L-selectin or LECAM-1, a 74-95 kDa type I transmembrane glycoprotein and cell adhesion molecule expressed on most circulating leukocytes¹. Ligands for CD62L include CD34, GlyCAM-1, and MAdCAM-1, which require sialyation, fucosylation, and carbohydrate sulfation for recognition^{2, 3}. Ligand binding mediates tethering and rolling of leukocytes, facilitating entry into secondary lymphoid organs, including lymph nodes and Peyer’s patches, via high endothelial venules (HEVs)¹. CD62L is also expressed on human embryo trophoblasts and has been proposed to play a role in human embryo implantation^4.

Antigen Details

Protein

CD62L

Ligand/Receptor

CD34, GlyCAM, MAdCAM-1

Function

Leukocyte homing, leukocyte tethering, rolling

PubMed

CD62L

NCBI Gene Bank ID

6402

UniProt.org

Information on Uniprot.org

Research Area

Cell Adhesion

.

Cell Biology

.

Costimulatory Molecules

.

Immunology

.

Innate Immunity

References & Citations

1. Ivetic A, et al. (2019) Front Immunol. 10:1068

2. Varki A (1994) Proc Natl Acad Sci USA. 91(16):7390-7397

3. Rosen SD. (1999) Am J Pathol. 155(4):1013-1020

4. Genbacev OD, et al. (1003) Science. 299(5605):405-8

Technical Protocols