Anti-Mouse CD4 (Clone GK1.5) - Biotin

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Product Concentration

0.5 mg/ml

Formulation

This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2829200

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this GK1.5 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

IHC-F

ICC

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.

Background

CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.¹

Antigen Details

Protein

CD4

Ligand/Receptor

MHC class II molecule

PubMed

CD4

NCBI Gene Bank ID

12504

UniProt.org

Information on Uniprot.org

Research Area

Immunology

References & Citations

1. Hendrickson WA et al. (1994) Structure 2: 59

Technical Protocols