Antibody Details
Product Details
Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CD5 (> 98%)
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Yellow Laser (563 nm)
RRID
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this UCHT-2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
IHC (Frozen)
WB
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Mouse Anti-Human CD5 (Clone UCHT-2) recognizes an epitope on Human CD5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and > 90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.
Background
Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.
Antigen Details
Protein
CD5
Ligand/Receptor
CD72
PubMed
CD5
NCBI Gene Bank ID
921
UniProt.org
Information on Uniprot.org
Research Area
Immunology
References & Citations
1. Leukocyte Typing VI (1995) Oxford University Press2. Leukocyte Typing III (1987) Oxford University Press3. Beverly, P. C. L. et al. (1981) "Protides of the Biological Fluids, " Vol. XXIX, Peeters, H. (ed.), Pergamon Press, Oxford, 653
Technical Protocols