Anti-Human CD22 - DyLight 594

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human CD22 (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 594 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 594 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Red Laser (590 nm)

RRID

AB_2829037

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this RFB-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human CD22 (Clone RFB-4) recognizes an epitope on Human CD22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

The CD22 antigen is expressed on > 90% of peripheral blood B-cells.¹ This antigen is not present on T-cells, monocytes or macrophages. CD22 is expressed on most peripheral B-cell lymphomas and leukemias including hairy cell leukemia, but not on the immature bone marrow type of leukemia.

Background

Cluster of differentiation 22 also known as CD22 is a B-cell-restricted transmembrane protein, which acts as a negative regulator of B-cell signalling. CD22 also has lectin-like adhesive properties.¹ CD22 is a good target to shorten the time required for Ab production, thereby augmenting host defense against acute infectious diseases as "universal vaccination."²

Antigen Details

Protein

CD22

Ligand/Receptor

CD45RO, CD75

PubMed

CD22

NCBI Gene Bank ID

933

UniProt.org

Information on Uniprot.org

Research Area

Cell Adhesion

.

Cell Biology

.

Immunology

References & Citations

1. Crocker PR et al. (2000) Immunol. 101: 342
2. Tsubata T et al. (2008) J Immunol. 180: 907

Technical Protocols