Anti-Human CD2 - DyLight 650

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human CD2 (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 650 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Red Laser (652 nm)

RRID

AB_2828906

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this G11 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone G11 recognizes an epitope of human CD2.

Antigen Distribution

CD2 is present on normal peripheral blood lymphocytes, thymocytes, mature circulating T-cells and a subset of NK cells.

Background

CD2 is a 45-59 kD type I transmembrane glycoprotein belonging to the Ig superfamily. CD2 is the receptor for LFA-3/CD58. CD2 serves as an adhesion receptor that binds to CD58; generating the activation of CD2-positive T cells and NK cells and in the regulation of their cytolytic activities. CD2 is a specific marker for T cells and NK cells. Hence, it can be used in immunohistochemistry to identify the occurrence of these cells in tissue sections. The vast majority of T cell lymphomas and leukemias express CD2 which makes it possible to distinguish these conditions from B-cell lymphomas.

Antigen Details

Protein

CD2

Ligand/Receptor

CD58 (LFA-3), CD48, CD59, CD15

PubMed

CD2

NCBI Gene Bank ID

914

UniProt.org

Information on Uniprot.org

Research Area

Costimulatory Molecules

.

Immunology

References & Citations

1. Meuer, S.C. et al. (1984) Cell 36:897

2. Peterson, A. and Sneed, B. (1987) Nature 329:842

3. Bierer, B.E. et al.(1989) Annu. Rev Immunol. 7:579

4. Krensky, C.F. et al. (1982) Proc. Natl. Acad. Sci. USA 79:7489

5. Krensky, C.F. et al. (1983) J. Immunol. 131:611

Technical Protocols