Antibody Details
Product Details
Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CASP3 (> 98%)
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Red Laser (652 nm)
RRID
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Mouse Anti-Human Caspase-3 Active Subunit (p17) (CASP3) (Clone C31.129) recognizes an epitope on Human CASP3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Caspase-3 is a member of the caspase family of cysteine proteases and plays a key role in mediating Fas (CD95) and tumor necrosis factor–1 (TNFR-1) induced apoptosis. Caspases can be divided into three groups: apoptotic initiators, apoptotic executioners, and inflammatory mediators.1, 2 Caspase-3 is activated during apoptotic signaling events by upstream proteases including caspase 6 and caspase 8.3 Caspase-3 is thought to be essential for chromatin margination, DNA fragmentation and nuclear collapse during apoptosis.3
Antigen Details
Protein
Caspase-3
PubMed
Caspase-3
NCBI Gene Bank ID
836
UniProt.org
Information on Uniprot.org
References & Citations
1. Adadah, BA. et al. (2000) Journal of Neurotoma 17(10): 811
2. Salvesen, GS. et al. (1999) Proc. Natl. Acad. Sci. USA 96(20): 10964
3. Slee, EA. et al. (2001) J. Biol. Chem. 276(10):7320
Technical Protocols