Antibody Details
Product Details
Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.2 mg/ml
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 3.9 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.>
Additional Reported Applications For Relevant Conjugates ?
IHC (Frozen)
FA
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Clone 3.9 recognizes the α-chain (CD11c) of the CD11c/CD18 complex. It is specific for the I domain of CD11c. Clone 3.9 binds the activated form of CD11c and partially blocks the binding of CD11c with ICAM-4.
Antigen Distribution
CD11c is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells.
Background
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
Antigen Details
Protein
CD11c
Ligand/Receptor
CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
PubMed
CD11c
NCBI Gene Bank ID
3687
UniProt.org
Information on Uniprot.org
Research Area
Cell Adhesion
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Cell Biology
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Costimulatory Molecules
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Immunology
.
Innate Immunity
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Neuroscience
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Neuroscience Cell Markers
References & Citations
1. Leukocyte Typing V (1995) Oxford University Press2. Leukocyte Typing IV (1989) Oxford University Press3. Leukocyte Typing III (1987) Oxford University Press4. Hogg, N. et al. (1986) Eur. J. Immunol. 16:2405. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117Phycobilliproteins are protected under the following patents: (U.S. patents No. 4, 520, 110 and 4, 542, 104, European patent no. 76695, Canadian patent No. 1, 179, 942 and Australian patent No. 548, 440).
Technical Protocols