Anti-Rat CNTF (Clone 34726)

Antibody Details

Product Details

Reactivity Species

Rat

Host Species

Mouse

Immunogen

E. coli-Derived Recombinant Rat CNTF

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2828503

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Rat CNTF this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rrCNTF is approximately 5 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Rat Ciliary Neurotrophic Factor (CNTF) (Clone 34726) recognizes an epitope on Rat CNTF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Ciliary neurotrophic factor (CNTF) is a cytokine¹ that supports survival and/or differentiation of a variety of neuronal cell types including sensory, sympathetic, and motoneurons.² With these abilities, CNTF has implications for Parkinson's disease and cell-replacement therapies for other disorders.³ CNTF mimics the biological actions of leptin while overcoming "leptin resistance" and appears to reduce inflammatory signaling cascades associated with lipid accumulation in liver and skeletal muscle. The advantages and disadvantages of CNTF has shown promise as a therapeutic strategy to alleviate obesity-associated diseases.⁴

Antigen Details

Protein

CNTF

PubMed

CNTF

NCBI Gene Bank ID

25707

UniProt.org

Information on Uniprot.org

References & Citations

1. Boschero, AC. et al. (2009) Cytokine 46(1):65-71

2. Thoenen, H. et al. (1994) J Neurobiol. 25: 1436

3. Hagg, T. et al. (2008) J Neurosci. 28: 2231

4. Febbraio, MA. et al. (2008) J Mol Med. 86: 353

Technical Protocols