Anti-Human 4-1BBR (CD137)

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human 4-1BB Receptor (> 98%) (Accession # Q07011)

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2828182

Applications and Recommended Usage?
Quality Tested by Leinco

FC It is recommended to use the indirect method for signal enhancement when enumerating cells expressing 4-1BB Receptor. A suggested method would be to stain cells expressing 4-1BB Receptor with 0.25 µg per 1 - 2.5 x 10⁵ cells in a total staining volume of ≤200 µl followed by PN:G641.
WB To detect Human 4-1BB Receptor this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human 4-1BB Receptor is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F156.
ELISA Sandwich This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.4 ng/ml. A suitable detection antibody is PN:B392 at a concentration of approximately 0.1 - 0.4 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is ~4 ng/well. A suggested standard for this assay is PN:F156.

Other Applications Reported In Literature ?

ICC In Immunocytochemistry, this antibody will detect 4-1BB in cells. The working dilution is 2 - 15 μg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human 4-1BB Receptor recognizes Human 4-1BB Receptor. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Human 4-1BB Receptor, a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells.

Antigen Details

Protein

4-1BBR

PubMed

4-1BBR

NCBI Gene Bank ID

3604

UniProt.org

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Technical Protocols