Item no. |
ICH1182-100MG |
Manufacturer |
IchorBio
|
Amount |
100 mg |
Quantity options |
100 mg
10 mg
1 mg
20 mg
25 mg
50 mg
5 mg
|
Category |
|
Type |
Antibody |
Applications |
WB, FC, Blocking, FA |
Clone |
RMP1-14 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Mouse |
Isotype |
IgG2a |
Purity |
>95% by SDS-PAGE and HPLC |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Programmed cell death protein 1, Pdcd1, CD279 |
Shipping condition |
Cool pack |
Available |
|
Specificity |
Mouse anti-PD-1 antibody (RMP1-14) is a recombinant antibody where the original Rat IgG2a backbone of clone RMP1-14 has been changed to Mouse IgG1. This antibody recognizes an epitope on Mouse PD-1 |
Manufacturer - Applications |
Western Blot, Blocking, Flow Cytometry, Functional Assays |
Manufacturer - Category |
Low Endotoxin, anti-mouse, monoclonal antibody |
Manufacturer - Isotype |
Mouse IgG2a |
Manufacturer - Targets |
PD-1 |
Shipping Temperature |
Blue ice |
Storage Conditions |
Mouse anti-mouse PD-1 antibody (RMP1-14) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC. |
Aggregation |
Aggregation level ≤ 5% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
Subset of double negative thymocytes, activated T and B cells |
Application Notes |
Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production. |
Concentration |
1.0 - 5.0 mg/ml |
Endotoxin |
≤ 1.0 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Mouse anti-PD-1 antibody (RMP1-14) is a recombinant antibody where the original Rat IgG2a backbone of clone RMP1-14 has been changed to Mouse IgG1. This antibody recognizes an epitope on Mouse PD-1 |
Purification method |
This monoclonal antibody was purified using Protein A |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunogen |
Mouse PD-1 transfected BHK cells |
Isotype Control |
Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247) |
Buffer |
ICH3001-100ml ICH3002-100ml ICH3003-100ml |