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Anti-PD-1 (RMP1-14) - Ultra-Low Endotoxin

Anti-PD-1 (RMP1-14) - Ultra-Low Endotoxin

Item no.	ICH1132UL-5MG
Manufacturer	IchorBio
Amount	5 mg
Quantity options	100 mg 25 mg 50 mg 5 mg
Category	Oncology Antibodies In Vivo Antibodies By Cell Type Carcinoma Hematology Lymphoma Myelofibrosis Western Blot Blocking
Type	Antibody
Applications	WB, FC, Blocking, FA
Clone	RMP1-14
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG2a Kappa
Purity	>98% by SDS-PAGE and HPLC
Citations	Chen et al. A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles. Nature Communications
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Programmed cell death protein 1, Pdcd1, CD279
Shipping condition	Cool pack
Available
Specificity	Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1
Manufacturer - Applications	Western Blot, Blocking, Flow Cytometry, Functional Assays
Manufacturer - Category	Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Rat IgG2a Kappa
Manufacturer - Targets	PD-1
Shipping Temperature	Blue ice
Storage Conditions	anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 1%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution	Subset of double negative thymocytes, activated T and B cells
Application Notes	Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required. Each investigator should determine their own optimal working dilution for specific applications.
Background	CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.
Concentration	≥ 2.0 mg/ml
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1
Purification method	This monoclonal antibody was purified using Protein G
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	Mouse PD-1 transfected BHK cells
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Seoul virus Mouse adenovirus Reovirus 3 Rat theilovirus
Related Antibodies	ICH1132-1MG
Isotype Control	Rat IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [1-1] (ICH2244UL)
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 5 mg

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Delivery expected until 2/7/2025

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