Item no. |
ICH1132UL-100MG |
Manufacturer |
IchorBio
|
Amount |
100 mg |
Quantity options |
100 mg
25 mg
50 mg
5 mg
|
Category |
|
Type |
Antibody |
Applications |
WB, FC, Blocking, FA |
Clone |
RMP1-14 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Rat |
Isotype |
IgG2a Kappa |
Purity |
>98% by SDS-PAGE and HPLC |
Citations |
Chen et al. A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles. Nature Communications |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Programmed cell death protein 1, Pdcd1, CD279 |
Shipping condition |
Cool pack |
Available |
|
Specificity |
Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1 |
Manufacturer - Applications |
Western Blot, Blocking, Flow Cytometry, Functional Assays |
Manufacturer - Category |
Ultra-Low Endotoxin, anti-mouse, monoclonal antibody |
Manufacturer - Isotype |
Rat IgG2a Kappa |
Manufacturer - Targets |
PD-1 |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
Aggregation |
Aggregation level ≤ 1% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
Subset of double negative thymocytes, activated T and B cells |
Application Notes |
Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required. Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production. |
Concentration |
≥ 2.0 mg/ml |
Endotoxin |
≤ 0.5 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1 |
Purification method |
This monoclonal antibody was purified using Protein G |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunogen |
Mouse PD-1 transfected BHK cells |
IMPACT Pathogen Test |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Seoul virus Mouse adenovirus Reovirus 3 Rat theilovirus |
Related Antibodies |
|
Isotype Control |
Rat IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [1-1] (ICH2244UL) |
Buffer |
ICH3001-100ml ICH3002-100ml ICH3003-100ml |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.