Anti-Mouse PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12)

PD-1

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.

≥ 2.0 mg/ml

≤ 0.5 EU/mg as determined by the LAL method

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: - Mycoplasma spp - Mycoplasma pulmonis - Pneumonia virus of mice - Kilham's rat virus - Toolan's H1 virus - Rat parvovirus - Lymphocytic choriomeningitis virus - Rat cytomegalovirus - Sendai virus - Rat coronavirus - Sialodacryoadenitis virus - Rat minute virus - Seoul virus - Mouse adenovirus - Reovirus 3 - Rat theilovirus

Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. - Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3, 6, 9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. - Each investigator should determine their own optimal working dilution for specific applications.