Anti-Mouse PD-1 (29F.1A12) In Vivo Antibody - Ultra-Low Endotoxin

Ultra-Low Endotoxin, anti-mouse, monoclonal antibody

PD-1

anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml.
Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3, 6, 9 @ 200 ug per animal per injection) then the tumor will regress, and disappear.
Each investigator should determine their own optimal working dilution for specific applications.

≥ 2.0 mg/ml

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus

Rat IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [1-1] (ICH2244UL)