Item no. |
ICH1091-5MG |
Manufacturer |
IchorBio
|
Amount |
5 mg |
Quantity options |
100 mg
1 mg
25 mg
50 mg
5 mg
|
Category |
|
Type |
Antibody |
Applications |
WB, FC, IHC, Blocking |
Clone |
29F.1A12 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Rat |
Isotype |
IgG2a Kappa |
Purity |
>95% by SDS-PAGE and HPLC |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Programmed cell death protein 1, Pdcd1, CD279 |
Shipping condition |
Cool pack |
Available |
|
Specificity |
Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
Manufacturer - Applications |
Flow Cytometry, Western Blot, Blocking, IHC (Frozen) |
Manufacturer - Category |
Low Endotoxin, anti-mouse, monoclonal antibody |
Manufacturer - Isotype |
Rat IgG2a Kappa |
Manufacturer - Targets |
PD-1 |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
Aggregation |
Aggregation level ≤ 5% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells |
Application Notes |
Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3, 6, 9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications. |
Background |
Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. |
Concentration |
1.0 - 5.0 mg/ml |
Endotoxin |
≤ 1.0 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
Purification method |
This monoclonal antibody was purified using Protein G |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunogen |
PD-1 cDNA followed by PD-1-Ig fusion protein |
Related Antibodies |
|
Isotype Control |
Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244) |
Buffer |
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml |