Item no. |
ICH1060-50MG |
Manufacturer |
IchorBio
|
Amount |
50 mg |
Quantity options |
100 mg
1 mg
25 mg
50 mg
5 mg
|
Category |
|
Type |
Antibody |
Applications |
WB, FC, IP, IHC, Blocking |
Clone |
MEL-14 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Rat |
Isotype |
IgG2a |
Purity |
>95% by SDS-PAGE and HPLC |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
L-selectin, Sell, Leukocyte adhesion molecule 1, LAM-1, Leukocyte-endothelial cell adhesion molecule 1, LECAM1<br />Lymph node homing receptor1, Lymphocyte antigen 221,<br />Ly-221, Lymphocyte surface MEL-14 antigen, Lnhr, Ly-22, Ly22 |
Shipping condition |
Cool pack |
Available |
|
Specificity |
Anti-CD62L In Vivo Antibody - Low Endotoxin (MEL-14) recognizes an epitope on Mouse CD62L |
Manufacturer - Applications |
Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, IHC (Frozen), CyTOF |
Manufacturer - Category |
Low Endotoxin, anti-mouse, monoclonal antibody |
Manufacturer - Isotype |
Rat IgG2a |
Manufacturer - Targets |
CD62L |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-CD62L In Vivo Antibody - Low Endotoxin (MEL-14) is stable for at least six (6) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
Aggregation |
Aggregation level ≤ 5% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
Subsets of B and T cells, monocytes, granulocytes, subset of NK cells |
Application Notes |
Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, and LAM-1. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody. |
Concentration |
1.0 - 5.0 mg/ml |
Endotoxin |
≤ 1.0 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Anti-CD62L In Vivo Antibody - Low Endotoxin (MEL-14) recognizes an epitope on Mouse CD62L |
Purification method |
This monoclonal antibody was purified using Protein G |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunogen |
C3H/eb mouse B lymphoma 38C-13 |
Antibodies against the same target (Cat No.) |
|
Isotype Control |
Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244) |
Buffer |
ICH3001-100ml ICH3002-100ml ICH3003-100ml |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.