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Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin

Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin

Item no.	ICH1056-5MG
Manufacturer	IchorBio
Amount	5 mg
Quantity options	100 mg 1 mg 25 mg 50 mg 5 mg
Category	Antibodies In Vivo Antibodies Western Blot Blocking
Type	Antibody
Applications	WB, FC, IP, IHC, Blocking
Clone	2.4G2
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG2b
Purity	>95% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Low affinity immunoglobulin gamma Fc region receptor II, Fcgr2, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Ly-17, Low affinity immunoglobulin gamma Fc region receptor III, Fcgr3, FcRIII, Fc-gamma RIII
Shipping condition	Cool pack
Available
Specificity	Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors
Manufacturer - Applications	Western Blot, Blocking, Flow cytometry, IHC (Frozen), Immunoprecipitation, CODEX
Manufacturer - Category	Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Rat IgG2b
Manufacturer - Targets	CD32/CD16
Shipping Temperature	Blue ice
Storage Conditions	anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 5%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution	The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes.
Application Notes	Each investigator should determine their own optimal working dilution for specific applications.
Background	CD16 is a low-affinity IgG Fc receptor III and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. Clone 2.4G2 antibody is used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen-specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo.
Concentration	0.5 mg/ml
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors
Purification method	This monoclonal antibody was purified using Protein G
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Antibodies against the same target (Cat No.)	ICH1010-1MG
Isotype Control	Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 5 mg

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Delivery expected until 2/7/2025

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