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Anti-CD3e (145-2C11) In Vivo Antibody - Ultra-Low Endotoxin

Anti-CD3e (145-2C11) In Vivo Antibody - Ultra-Low Endotoxin

Item no.	ICH1041UL-25MG
Manufacturer	IchorBio
Amount	25 mg
Quantity options	100 mg 25 mg 50 mg 5 mg
Category	In Vivo Antibodies In Vivo Antibodies Flow Cytometry Immunohistochemistry Western Blot Blocking
Type	Antibody
Applications	WB, FC, IP, IHC, Blocking, IV
Clone	145-2C11
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Isotype	IgG
Purity	>98% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e
Shipping condition	Cool pack
Available
Specificity	Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e
Manufacturer - Applications	IHC (Frozen), Flow Cytometry, Immunoprecipitation, Western Blot, In vivo Depletion, Blocking
Manufacturer - Category	Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Armenian Hamster IgG
Manufacturer - Targets	CD3e
Shipping Temperature	Blue ice
Storage Conditions	anti-CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 1%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution	The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.
Application Notes	Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells Each investigator should determine their own optimal working dilution for specific applications.
Background	CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Concentration	1.0 - 5.0 mg/ml
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e
Purification method	This monoclonal antibody was purified using Protein A
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Hamster parvovirus Lymphocytic choriomeningitis Minute virus of mice Theiler's murine encephalomyelitis virus Sendai virus Reovirus 3 Hantaan virus
Related Antibodies	ICH1041-1MG
Antibodies against the same target (Cat No.)	ICH1040UL-5MG, ICH1040-1MG
Isotype Control	Armenian Hamster IgG Isotype Control for In Vivo - Ultra Low Endotoxin [PIP] (ICH2251UL)
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 25 mg

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Delivery expected until 2/7/2025

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