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Anti-Mouse CD3e In Vivo Antibody - Low Endotoxin (145-2C11)

Anti-Mouse CD3e In Vivo Antibody - Low Endotoxin (145-2C11)

Item no.	ICH1041-25MG
Manufacturer	IchorBio
Amount	25mg
Category	Antibodies In Vivo Antibodies Blocking
Type	Antibody
Applications	WB, FC, IP, IHC, Blocking, IV
Clone	145-2C11
Specific against	Mouse
Host	Hamster - Armenian
Isotype	IgG
Purity	>95% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e
Available
Specificity	Anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e
Manufacturers Product Category	Low Endotoxin, anti-mouse
Isotype Information	Armenian Hamster IgG
Purification method	This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution	The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.
Background	CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Immunogen	H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Concentration	1.0 mg/ml
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Aggregation	Aggregation level ≤ 5%
Storage	anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Application Notes	Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells - Each investigator should determine their own optimal working dilution for specific applications.
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 25mg

available

Delivery expected until 12/20/2024