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Anti-Mouse CD3e In Vivo Antibody - Low Endotoxin (145-2C11) European Partner

Item no. ICH1041-100MG
Manufacturer IchorBio
Amount 100mg
Category
Type Antibody
Applications WB, FC, IP, IHC, Blocking, IV
Clone 145-2C11
Specific against Mouse
Host Hamster - Armenian
Isotype IgG
Purity >95% by SDS-PAGE and HPLC
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e
Available
Specificity Anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e
Manufacturers Product Category
Low Endotoxin, anti-mouse
Isotype Information
Armenian Hamster IgG
Purification method
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.
Background
CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Concentration
1.0 mg/ml
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Aggregation
Aggregation level ≤ 5%
Storage
anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Application Notes
Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells - Each investigator should determine their own optimal working dilution for specific applications.
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100mg
Available: In stock
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