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Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin

Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin

Item no.	ICH1040-50MG
Manufacturer	IchorBio
Amount	50 mg
Quantity options	100 mg 1 mg 25 mg 50 mg 5 mg
Category	Antibodies In Vivo Antibodies
Type	Antibody
Applications	FC, IHC, FA
Clone	500A2
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Syrian
Isotype	IgG2a Kappa
Purity	>95% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e
Shipping condition	Cool pack
Available
Specificity	Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) recognizes an epitope on Mouse CD3ε
Manufacturer - Applications	Functional Assays, Flow Cytometry, IHC (Frozen)
Manufacturer - Category	Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Syrian Hamster IgG2a Kappa
Manufacturer - Targets	CD3e
Shipping Temperature	Blue ice
Storage Conditions	anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 5%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution	The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.
Application Notes	Functional Assay: Binding of Hamster Anti-Mouse CD3ε (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. Each investigator should determine their own optimal working dilution for specific applications.
Background	CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex.
Concentration	1.0 mg/ml
Endotoxin	<1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) recognizes an epitope on Mouse CD3ε
Purification method	This monoclonal antibody was purified using Protein A
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	Mouse T-cell receptor
Related Antibodies	ICH1040UL-5MG
Antibodies against the same target (Cat No.)	ICH1041UL-5MG, ICH1041-1MG
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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