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Anti-CD86 (GL1) In Vivo Antibody - Low Endotoxin

Anti-CD86 (GL1) In Vivo Antibody - Low Endotoxin

Item no.	ICH1038-100MG
Manufacturer	IchorBio
Amount	100 mg
Quantity options	100 mg 1 mg 25 mg 50 mg 5 mg
Category	Antibodies In Vivo Antibodies Western Blot Blocking
Type	Antibody
Applications	WB, FC, IF, IP, IHC, ICC, Blocking
Clone	GL1
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG2a Kappa
Purity	>95% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1
Shipping condition	Cool pack
Available
Specificity	Anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) recognizes an epitope on Mouse CD86
Manufacturer - Applications	Flow Cytometry, Immunoprecipitation, IHC (Frozen), Immunocytochemistry, Blocking, Immunofluorescence, Western Blot, CyTOF
Manufacturer - Category	Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Rat IgG2a Kappa
Manufacturer - Targets	CD86
Shipping Temperature	Blue ice
Storage Conditions	anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 5%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution	B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Application Notes	Each investigator should determine their own optimal working dilution for specific applications.
Background	CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the Ig gene superfamily. CD86 is a ligand for CD28 and CD152 (CTLA-4). CD86 is related to the CD80 molecule and functions as a counterreceptor for CD28 and CTLA-4. The GL1 clone has been shown to provide critical signals for T-Cell-dependent responses in vivo and in vitro.
Concentration	1.0 mg/ml
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) recognizes an epitope on Mouse CD86
Purification method	This monoclonal antibody was purified using Protein G
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	LPS-activated CBA/Ca mouse splenic B cells
Isotype Control	Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 mg

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Delivery expected until 2/7/2025

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