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Bulk Anti-Human CD16 (3G8) Antibody

Bulk Anti-Human CD16 (3G8) Antibody

Item no.	ICH1010-5MG
Manufacturer	IchorBio
Amount	5 mg
Quantity options	100 mg 1 mg 25 mg 50 mg 5 mg
Category	Antibodies In Vivo Antibodies Flow Cytometry Immunohistochemistry Blocking
Type	Antibody
Applications	FC, IP, IHC, Blocking
Clone	3G8
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Purity	>95% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	FCGR3A, Fc-gamma RIII, FcRIII
Shipping condition	Cool pack
Available
Specificity	Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16
Manufacturer - Applications	IHC (Frozen), Flow Cytometry, Immunoprecipitation, Blocking, CyTOF
Manufacturer - Category	Low Endotoxin, anti-human, monoclonal antibody
Manufacturer - Isotype	Mouse IgG1 Kappa
Manufacturer - Targets	CD16
Shipping Temperature	Blue ice
Storage Conditions	anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is stable when stored at 2-8°C.
Aggregation	Aggregation level ≤ 5%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide added.
Antigen Distribution	CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans.
Application Notes	Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Each investigator should determine their own optimal working dilution for specific applications.
Background	CD16 is known as low affinity IgG receptor III (Fc gamma RIII) and is expressed as two distinct forms (CD16a and CD16b). CD16a (Fc gamma RIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (Fc gamma RIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils.
Concentration	≥ 2.0 mg/ml
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16
Purification method	This monoclonal antibody was purified using Protein A
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	Human PMN cells
Antibodies against the same target (Cat No.)	ICH1056-1MG
Isotype Control	Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 5 mg

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Delivery expected until 2/7/2025

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