Item no. |
ICH1010-50MG |
Manufacturer |
IchorBio
|
Amount |
50 mg |
Quantity options |
100 mg
1 mg
25 mg
50 mg
5 mg
|
Category |
|
Type |
Antibody |
Applications |
FC, IP, IHC, Blocking |
Clone |
3G8 |
Specific against |
Human (Homo sapiens) |
Host |
Mouse |
Isotype |
IgG1 Kappa |
Purity |
>95% by SDS-PAGE and HPLC |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
FCGR3A, Fc-gamma RIII, FcRIII |
Shipping condition |
Cool pack |
Available |
|
Specificity |
Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16 |
Manufacturer - Applications |
IHC (Frozen), Flow Cytometry, Immunoprecipitation, Blocking, CyTOF |
Manufacturer - Category |
Low Endotoxin, anti-human, monoclonal antibody |
Manufacturer - Isotype |
Mouse IgG1 Kappa |
Manufacturer - Targets |
CD16 |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is stable when stored at 2-8°C. |
Aggregation |
Aggregation level ≤ 5% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide added. |
Antigen Distribution |
CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. |
Application Notes |
Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD16 is known as low affinity IgG receptor III (Fc gamma RIII) and is expressed as two distinct forms (CD16a and CD16b). CD16a (Fc gamma RIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (Fc gamma RIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. |
Concentration |
≥ 2.0 mg/ml |
Endotoxin |
≤ 1.0 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16 |
Purification method |
This monoclonal antibody was purified using Protein A |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunogen |
Human PMN cells |
Antibodies against the same target (Cat No.) |
|
Isotype Control |
Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247) |
Buffer |
ICH3001-100ml ICH3002-100ml ICH3003-100ml |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.