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Bulk anti-Human CD16 antibody (3G8)

Bulk anti-Human CD16 antibody (3G8)

Manufacturer	IchorBio
Category	Antibodies In Vivo Antibodies Flow Cytometry Immunohistochemistry Blocking
Type	Antibody
Specific against	Human
Clone	3G8
Isotype	IgG1 Kappa
Applications	FC, IP, IHC, Blocking
Amount	100mg
Host	Mouse
Item no.	ICH1010-100MG
eClass 6.1	32160702
eClass 9.0	32160702
Available
Alias	FCGR3A, Fc-gamma RIII, FcRIII
Purity	>95% by SDS-PAGE and HPLC
Manufacturers Product Category	Low Endotoxin, anti-human
Target Information	CD16
Isotype Information	Mouse IgG1 Kappa
Purification method	This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution	CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans.
Background	CD16 is known as low affinity IgG receptor III (Fc gamma RIII) and is expressed as two distinct forms (CD16a and CD16b). CD16a (Fc gamma RIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (Fc gamma RIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils.
Immunogen	Human PMN cells
Concentration	≥ 2.0 mg/ml
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide added.
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Aggregation	Aggregation level ≤ 5%
Storage	anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is stable when stored at 2-8°C.
Application Notes	Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. - Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. - Each investigator should determine their own optimal working dilution for specific applications.
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100mg

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Delivery expected until 8/2/2024

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