Item no. |
FNab05372-100ug |
Manufacturer |
FineTest
|
Amount |
100ug |
Category |
|
Type |
Antibody Monoclonal |
Format |
liquid |
Clone |
7C1 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG2a |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2 |
Available |
|
Purification |
Protein A+G purification |
Purity |
>=95% as determined by SDS-PAGE |
Clonality |
monoclonal |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20C for 12 months(Avoid repeated freeze / thaw cycles.) |
Background |
Component of the post-replicative DNA mismatch repair system(MMR). Forms two different heterodimers: MutS alpha(MSH2-MSH6 heterodimer) and MutS beta(MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops(IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. |
Immunogen |
mutS homolog 2, colon cancer, nonpolyposis type 1 |
Observed MW |
105 kDa |
Uniprot ID |
P43246 |
Tested Application |
ELISA, WB, IHC |
Recommended dilution |
WB: 1:500-1:2000; IHC: 1:20-1:200 |
Gene ID |
4436 |
Research Area |
Epigenetics, Metabolism, Cancer, Immunology, Developmental biology |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.