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AF/LE Purified Anti-Mouse F4/80 Antibody[CI:A3-1]

AF/LE Purified Anti-Mouse F4/80 Antibody[CI:A3-1]

Item no.	E-AB-F09950-50
Manufacturer	Elabscience
Amount	50 ug
Quantity options	1 mg 100 mg 100 ug 25 mg 25 ug 50 ug 500 ug 5 mg
Category	Antibodies Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies Functional Assays
Type	Antibody Monoclonal
Applications	FC, FA
Clone	CI:A3-1
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG2b
Conjugate/Tag	Unconjugated
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Adhesion G protein-coupled receptor E1, Adgre1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, Adgre1, Emr1, Gpf480,
Similar products	Emr1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1, Cell surface glycoprotein F4/80, Gpf480
Shipping condition	Cool pack
Available
Manufacturer - Applications	FCM, Depletion, FA
Manufacturer - Category	Flow Cytometry Antibodies
Manufacturer - Isotype	Rat IgG2b, κ
Manufacturer - Conjugate / Tag	None (AF/LE)
Shipping Temperature	Ice bag
Storage Conditions	Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Background	F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
Concentration	≥1 mg/mL
Buffer	Sterile PBS. < 1.0 EU per mg of the antibody as determined by the LAL method.
Abbreviation	F4/80
Clonality	Monoclonal
Usage	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10^6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 ug

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Delivery expected until 6/5/2025

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