Item no. |
CSB-PA118316 |
Manufacturer |
Cusabio
|
Amount |
100ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
4E-BP1 antibody; 4EBP1 antibody; 4EBP1_HUMAN antibody; BP 1 antibody; eIF4E binding protein 1 antibody; eIF4E-binding protein 1 antibody; Eif4ebp1 antibody; Eukaryotic translation initiation factor 4E-binding protein 1 antibody; PHAS-I antibody; PHASI antibody; Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody, EIF4EBP1, PHAS-1, |
Similar products |
EIF4EBP1, PHAS-1 |
Available |
|
Immunogen Species |
Human |
UniprotID |
Q13541 |
Immunogen |
Peptide sequence around aa.34ca.38 (S-T-T-P-G) derived from Human 4E-BP1. |
Applications info |
ELISA, WB, IHC, WB:1:500-1:1000, IHC:1:50-1:200 |
Dilution ratio 1 |
ELISA:1:2000-1:10000 |
Dilution ratio 2 |
WB:1:500-1:1000 |
Dilution ratio 3 |
IHC:1:50-1:200 |
Background |
4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Gingras AC, et al. Genes Dev Nov. 2005 Feb 15. Mothe-Satney I, et al.(2000) J Biol Chem:15(21): 2852-64. Gingras AC, et al. (1999) Genes Dev:13(11): 1422-37. Lal L, et al.(2005) Blood, 105(4): 1669-7. |
Conjugate |
Non-conjugated |
Buffer |
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage |
Upon receipt, store at -20C or -80C. Avoid repeated freeze. |
Purification Method |
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
General Research Areas |
Epigenetics and Nuclear Signaling, Metabolism |
Initial Research Areas |
Epigenetics and Nuclear Signaling |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.