Item no. |
BS94090-100 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
100ul |
Category |
|
Type |
Antibody Primary |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
cRaf, RAF1, c Raf, C-raf, C-Raf proto-oncogene, serine/threonine kinase, CMD1NN, Craf 1 transforming gene, cRaf, Craf1 transforming gene, EC 2.7.11.1, kinase Raf1, Murine sarcoma 3611 oncogene 1, NS5, Oncogene MIL, Oncogene RAF1, OTTHUMP00000160218, OTTHU |
Available |
|
Swiss-Prot |
P04049(Human) Q99N57(Mouse) P11345(Rat) |
Application_all |
WB:1:1, 000-1:2, 000 IHC:1:50-1:200 |
Background |
Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338. |
Product |
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
Purification And Purity |
ProA affinity purified |
Storage And Stability |
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles. |
Specificity |
Raf1 (Phospho-S259) polyclonal antibody detects endogenous levels of Raf1 protein only when phosphorylated at S259. |
BiowMW |
73 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Immunogen |
Synthetic phospho-peptide corresponding to residues surrounding Ser259 of human Raf1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.