Item no. |
BS91149-50 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
50ul |
Category |
|
Type |
Antibody Primary |
Applications |
WB, IF, ICC |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
cRaf, c Raf, C-raf, C-Raf proto-oncogene, serine/threonine kinase, CMD1NN, Craf 1 transforming gene, cRaf, Craf1 transforming gene, EC 2.7.11.1, kinase Raf1, Murine sarcoma 3611 oncogene 1, NS5, Oncogene MIL, Oncogene RAF1, OTTHUMP00000160218, OTTHUMP0000 |
Available |
|
Swiss-Prot |
P04049(Human) Q99N57(Mouse) P11345(Rat) |
Application_all |
WB:1:1, 000-1:2, 000 ICC:1:50-1:200 |
Background |
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1. |
Product |
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
Purification And Purity |
ProA affinity purified |
Storage And Stability |
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles. |
Specificity |
Raf1 polyclonal antibody detects endogenous levels of Raf1 protein. |
BiowMW |
73 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Immunogen |
recombinant protein |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.