Item no. |
BS90743-50 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
50ul |
Category |
|
Type |
Antibody Primary |
Applications |
WB, IF, IHC, ICC |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
IRS-1, IRS1, HIRS 1, HIRS1, Insulin receptor substrate 1, IRS 1, IRS-1, IRS1, IRS1, OTTHUMP00000164234, |
Available |
|
Swiss-Prot |
P35568(Human) |
Application_all |
WB:1:1, 000-1:2, 000 ICC:1:50-1:200 IHC:1:50-1:200 |
Background |
The insulin receptor substrate-1 (IRS-1), a protein major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/ threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS-1. |
Product |
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
Purification And Purity |
ProA affinity purified |
Storage And Stability |
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles. |
Specificity |
IRS1 polyclonal antibody detects endogenous levels of IRS1 protein. |
BiowMW |
170 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Immunogen |
recombinant protein |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.