Item no. |
BOS-A08476-1 |
Manufacturer |
Boster
|
Amount |
100 ug/vial |
Category |
|
Type |
Antibody |
Format |
Lyophilized |
Applications |
WB, IF, ICC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Short Description |
Boster Bio Anti-NPL4/NPLOC4 Antibody Picoband™ catalog # A08476-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-NPL4/NPLOC4 Antibody Picoband™ catalog # A08476-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Nuclear protein localization protein 4 homolog is a protein that in humans is encoded by the NPLOC4 gene. Nuclear protein localization protein 4 (NPL4, NPLOC4) was originally identified as a yeast nuclear transport protein that was later recognized as a critical component of the endoplasmic reticulum-associated degradation (ERAD) pathway. Mammalian NPL4 protein has an amino-terminal ubiquitin-like domain containing a p97 binding site, and a conserved carboxy-terminal zinc finger (NZF) motif responsible for binding ubiquitinated target proteins. NPL4 binds ubiquitin fusion degradation protein 1 (UFD1) to form a heterodimer that associates with the p97 AAA-ATPase, creating a protein complex that mediates delivery of ubiquitinated ER proteins to the proteasome. |
Gene Name |
NPLOC4 |
Application Details |
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Direct ELISA, 0.1-0.5 μg/ml, Human |
Application Notes |
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
Immunogen |
E.coli-derived human NPL4/NPLOC4 recombinant protein (Position: M1-N495). |
Purification |
Immunogen affinity purified. |
Storage |
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Reconstitution |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Molecular Weight |
72 kDa |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.