Item no. |
BM-CAU28688-200ul |
Manufacturer |
Biomatik
|
Amount |
200ul |
Category |
|
Type |
Antibody |
Applications |
WB, IP, IHC, ICC |
Specific against |
Guinea Pig |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1 |
Available |
|
Estimated Turnaround Time |
7-11 business days |
Target Name |
11-Beta-Hydroxysteroid Dehydrogenase Type 1 |
Target Synonyms |
11DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; SDR26C1; Corticosteroid 11-beta-dehydrogenase isozyme 1; Short Chain Dehydrogenase/Reductase Family 26C, Member 1 |
Sample Type |
Tissue homogenates, cell lysates and other biological fluids |
Detection Range |
1.56-100 ng/mL |
Sensitivity |
0.56 ng/mL |
Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%. |
Detection |
Colorimetric |
Assay Time |
3 hours |
Assay Type |
Double-antibody Sandwich |
Shipping Condition |
Ice packs |
Storage |
Short term: 4C; Long term: see manual. |
Precaution of Use |
The Stop Solution is acidic. Do not allow to contact skin or eyes. |
Expiry Date |
8 months |
UniProt ID |
P28845 |
Specificity |
This assay has high sensitivity and excellent specificity for detection of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). |
Cross Activity |
This assay has high sensitivity and excellent specificity for detection of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). |
Test Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Assay Procedure |
1. Prepare all reagents, samples and standards; 2. Add 100 uL standard or sample to each well. Incubate 2 hours at 37C; 3. Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37C; 4. Aspirate and wash 3 times; 5. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37C; 6. Aspirate and wash 5 times; 7. Add 90 uL Substrate Solution. Incubate 10-20 minutes at 37C; 8. Add 50 uL Stop Solution. Read at 450nm immediately. |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Restrictions |
For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals. |
Quality Systems |
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities. |
Quality Assurance |
Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in the kit before experiment. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.