Item no. |
ANOG-MO-P40003B |
Manufacturer |
Anogen
|
Amount |
0.5mg |
Category |
|
Type |
Antibody Monoclonal |
Format |
Aff. Pur |
Applications |
ELISA |
Clone |
B1C4 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG2 kappa |
Protein Family |
Signal Transduction |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Similar products |
CREB [p-S133] |
Available |
|
Description |
Mouse monoclonal antibody against serine133 phosphorylated human cAMP response element-binding protein (p133-CREB) |
Target Protein |
Serine133 phosphorylated human cAMP response element-binding protein (p133-CREB). |
Immunogen |
Serine133 phosphorylated human CREB peptide fragment conjugated with KLH |
Specificity |
Reactive to serine133 phosphorylated human CREB peptide fragment, and reactive to KLH or BSA conjugated serine133 phosphorylated human CREB peptide fragment. Not reactive to un-conjugated KLH or BSA. |
Purification |
Protein G affinity purified |
Species Reactivity |
Human, others not tested |
Myeloma |
Sp2/0-Ag14 |
Background |
cAMP response element-binding protein (CREB) is a transcription factor that regulates the transcription of a wide spectrum of genes in response to second massagers (cAMP and Ca++) and cell growth or stress signals. The primary binding sites of CREB on the promoter regions are cAMP response element (CRE) and calcium response element which have a common 5’-TGACGTCA-3’ motif. CREB can be phosphorylated and activated by cAMP-dependent protein kinase A, calcium/calmodulin-dependent kinase, and kinases in the mitogen-activated kinase (MAPK) and extracelluar signal-regulated kinase (ERK) signal cascades. CREB plays an important role in neuroplasticity and memory formation by controlling the expression of c-fos, brain-derived neurotrophic factor, tyrosine hydrolase and many neuropeptides. |
Formulation |
Lyophilized from a solution of 0.01M PBS, pH7.0 |
Reconstitution |
Double distilled water is recommended to adjust the final concentration to 1.00mg/mL. |
Application info |
In indirect ELISA, the antibody is reactive to serine133 phosphorylated human CREB peptide and reactive to serine133 phosphorylated human CREB peptide conjugated with KLH or BSA. The peptide and the conjugates were coated at 1?g/ml in pH 9.5 carbonate buffer. Other applications have not been evaluated. |
Research Area |
Signal transduction, gene transcription |
Storage |
Store at -20C |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.