Item no. |
ANOG-MO-I40018Q |
Manufacturer |
Anogen
|
Amount |
0.5mg |
Category |
|
Type |
Antibody Monoclonal |
Format |
Aff. Pur |
Applications |
WB, ELISA |
Clone |
133 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG1 kappa |
Protein Family |
Infectious Agents |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Similar products |
HCV |
Available |
|
Description |
Mouse monoclonal antibody to human hepatitis virus C (HCV) non-structural protein NS-3 |
Target Protein |
Human hepatitis C virus (HCV) non-structural protein NS-3 |
Immunogen |
Purified recombinant chimeric HCV polyprotein (555 amino acid residues) |
Specificity |
React with recombinant NS-3 protein (residues 1252-1477 on HCV polyprotein) and recombinant chimeric HCV polyprotein (60 kDa) |
Purification |
Protein G affinity purified |
Species Reactivity |
Human hepatitis C virus, others not tested |
Cross Reactivity |
No cross reaction was found with synthetic recombinant core protein C + envelope protein M (residues 1-142 on HCV polyprotein), synthetic core protein C (residues 1 -61), synthetic NS-3 protein (residues 1378-1458) and synthetic NS-4a protein (residues 1689-1735). |
Myeloma |
Sp2/0-Ag14 |
Background |
Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B. |
Formulation |
Lyophilized from a solution in 0.01M PBS, pH 7.0 |
Reconstitution |
Double distillated water is recommended to adjust the final concentration to 1.00mg/mL. |
Application info |
ELISA: React with HCV NS-3. Western Blot: mAb 133, when used at 0.1-1ug/mL, will allow visualization of 0.1ug ug/lane of recombinant NS-3 protein and 0.1ug/lane recombinant chimeric HCV polyprotein. |
Research Area |
Virology |
Storage |
Store at -20C |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.