Comparison

Monocyte Chemotactic Protein-1 (MCP-1/MCAF)

Item no. ANOG-MO-C40021D
Manufacturer Anogen
Amount 0.5mg
Category
Type Antibody Monoclonal
Format Aff. Pur
Applications WB, ELISA, NT
Clone S101
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 kappa
Protein Family Cytokine, Growth Factors
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Similar products MCP-1/MCAF
Available
Description
Mouse monoclonal antibody to human Monocyte Chemotactic Protein-1 (MCP-1)/ Monocyte Chemotactic and Activating Factor (MCAF)
Target Protein
Human MCP-1
Immunogen
Purified recombinant human MCP-1
Specificity
This antibody reacts with natural and recombinant human MCP-1.
Purification
Protein G affinity purified
Species Reactivity
Human, other species not tested
Cross Reactivity
This antibody does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1beta (IL-1beta), serum amyloid A (SAA) and epidermal growth factor (EGF).
Myeloma
Sp2/0-Ag14
Background
Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displays chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, auto-immune diseases, renal diseases, chronic infection and granuloma formation.
Formulation
Lyophilized from a solution in 0.01M PBS, pH 7.2
Reconstitution
Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
Application info
ELISA: The antibody can be used for quantitative detection of human MCP-1 in sandwich ELISA in combination with capture antibody S14.
Western Blot: 0.1ug/lane of recombinant human MCP-1 can be detected when antibody is used at 0.1-1ug/mL.
Neutralizing: The antibody at a concentration of 0.5ug/mL, neutralized 1nM recombinant human MCP-1 induced monocyte chemotaxis in blind well chemotaxis chambers.
Research Area
Cytokine, chemotaxis, inflammation
Storage
Store at -20C
Reference
Randolph, G. J. and Furie, M. B. A soluble gradient of endogenous monocyte chemoattractant protein-1 promotes the transendothelial migration of monocytes in vitro. J. Immunol. 1995 155:3610-3618.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.5mg
Available: In stock
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