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Monocyte Chemotactic Protein-1 (MCP-1/MCAF)

Monocyte Chemotactic Protein-1 (MCP-1/MCAF)

Item no.	ANOG-MO-C40021B
Manufacturer	Anogen
Amount	0.5mg
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Antibodies Western Blot ELISA Lipid & Fatty Acid Transport Primary Antibodies By Organ Heart & Blood Cancer Neutralization
Type	Antibody Monoclonal
Format	Aff. Pur
Applications	WB, ELISA, NT
Clone	S14
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 kappa
Protein Family	Cytokine, Growth Factors
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Similar products	MCP-1/MCAF
Available
Description	Mouse monoclonal antibody to human Monocyte Chemotactic Protein-1 (MCP-1)/ Monocyte Chemotactic and Activating Factor (MCAF)
Target Protein	Human MCP-1
Immunogen	Purified recombinant human MCP-1
Specificity	This antibody reacts with natural and recombinant human MCP-1.
Purification	Protein G affinity purified
Species Reactivity	Human, other species not tested
Cross Reactivity	This antibody does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1beta (IL-1beta), serum amyloid A (SAA) and epidermal growth factor (EGF).
Myeloma	Sp2/0-Ag14
Background	Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displays chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, auto-immune diseases, renal diseases, chronic infection and granuloma formation.
Formulation	Lyophilized from a solution in 0.01M PBS, pH 7.2
Reconstitution	Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
Application info	ELISA: This antibody can be used as a capture antibody in sandwich ELISA for human MCP-1 detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: MO-C40021T or Biotin-conjugated, Cat. No.: MO-C40021TB). Approximately 1.5pg/mL of MCP-1 in serum/plasma or 4pg/mL of MCP-1 in medium can be detected with an assay range of 0-1600pg/mL. Neutralizing: The antibody (clone S14) at a concentration of 0.5ug/mL, neutralized 1nM recombinant human MCP-1 induced monocyte chemotaxis in blind well chemotaxis chambers. It was also found that S-14 could inhibit the activity of native MCP-1 at concentrations similar to inhibitory doses for recombinant MCP-1. Western Blot: 0.1ug/lane of recombinant human MCP-1 can be detected when antibody is used at 0.1-1ug/mL.
Research Area	Cytokine, chemotaxis, inflammation
Storage	Store at -20C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.5mg

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Delivery expected until 2/21/2025

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