Item no. |
ANOG-MO-C40017G |
Manufacturer |
Anogen
|
Amount |
0.5mg |
Category |
|
Type |
Antibody Monoclonal |
Format |
Aff. Pur |
Applications |
WB, ELISA, NT |
Clone |
807 |
Specific against |
Human (Homo sapiens) |
Host |
Mouse |
Isotype |
IgG1 kappa |
Protein Family |
Cytokine, Growth Factors |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Similar products |
IL-8 |
Available |
|
Description |
Mouse monoclonal antibody to human interleukin 8 (IL-8) |
Target Protein |
Human IL-8 |
Immunogen |
Purified recombinant human IL-8 |
Specificity |
This antibody recognizes both recombinant and native human IL-8. |
Purification |
Protein G affinity purified |
Species Reactivity |
Human, other species not tested |
Cross Reactivity |
This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted). |
Myeloma |
Sp2/0-Ag14 |
Background |
Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis. |
Formulation |
Lyophilized from a solution in 0.01M PBS, pH 7.0 |
Reconstitution |
Double distilled water is recommended to adjust the final concentration to 1.00mg/mL |
Application info |
ELISA: React with human IL-8. Matched pair antibodies are available. Neutralizing: In chemotaxis assay, the antibody inhibited 95.1% of the chemotactic activity of IL-8 on RB/293 cell. 50ug/mL of antibody and 10ng/ml of human IL-8 were used in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the neutralizing activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes. Western Blot: The antibody when used at concentration of 0.02-0.1 ug/ml will allow visualization of 100ng/lane of human IL-8. |
Research Area |
Cytokine, interleukin and chemotaxis |
Storage |
Store at -20C |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.