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Interleukin-8 (IL-8)

Interleukin-8 (IL-8)

Item no.	ANOG-MO-C40017D
Manufacturer	Anogen
Amount	0.5mg
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Antibodies By Cell Type Carcinoma Western Blot ELISA Primary Antibodies By Organ Heart & Blood Cancer Neutralization
Type	Antibody Monoclonal
Format	Aff. Pur
Applications	WB, ELISA, NT
Clone	I8-60
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 kappa
Protein Family	Cytokine, Growth Factors
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Similar products	IL-8
Available
Description	Mouse monoclonal antibody to human interleukin 8 (IL-8)
Target Protein	Human IL-8
Immunogen	Purified recombinant human IL-8
Specificity	This antibody recognizes both recombinant and native human IL-8.
Purification	Protein G affinity purified
Species Reactivity	Human, other species not tested
Cross Reactivity	This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Myeloma	P3X63Ag8.653
Background	Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis.
Formulation	Lyophilized from a solution in 0.01M PBS, pH 7.0
Reconstitution	Double distilled water is recommended to adjust the final concentration to 1.00mg/mL
Application info	ELISA: This antibody can be used as a tracer antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: MO-C40017A). Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5ug/mL of antibody and 10ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody’s ability to neutralize IL-8 activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes. Western Blot: Concentration of 0.02-0.1ug/mL antibody will allow visualization of 100ng/lane of human IL-8.
Research Area	Cytokine, interleukin and chemotaxis
Storage	Store at -20C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.5mg

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Delivery expected until 2/21/2025

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