Comparison

Interleukin-8 (IL-8)

Item no. ANOG-MO-C40017D
Manufacturer Anogen
Amount 0.5mg
Category
Type Antibody Monoclonal
Format Aff. Pur
Applications WB, ELISA, NT
Clone I8-60
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 kappa
Protein Family Cytokine, Growth Factors
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Similar products IL-8
Available
Description
Mouse monoclonal antibody to human interleukin 8 (IL-8)
Target Protein
Human IL-8
Immunogen
Purified recombinant human IL-8
Specificity
This antibody recognizes both recombinant and native human IL-8.
Purification
Protein G affinity purified
Species Reactivity
Human, other species not tested
Cross Reactivity
This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Myeloma
P3X63Ag8.653
Background
Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis.
Formulation
Lyophilized from a solution in 0.01M PBS, pH 7.0
Reconstitution
Double distilled water is recommended to adjust the final concentration to 1.00mg/mL
Application info
ELISA: This antibody can be used as a tracer antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: MO-C40017A).
Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5ug/mL of antibody and 10ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody’s ability to neutralize IL-8 activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.
Western Blot: Concentration of 0.02-0.1ug/mL antibody will allow visualization of 100ng/lane of human IL-8.
Research Area
Cytokine, interleukin and chemotaxis
Storage
Store at -20C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.5mg
Available: In stock
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