Item no. |
ANOG-AI60001 |
Manufacturer |
Anogen
|
Amount |
1.0mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Purified |
Applications |
WB, ELISA, NT |
Specific against |
Human (Homo sapiens) |
Host |
Chicken |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
IL-8 |
Available |
|
Description |
Chicken anti-human Interleukin-8 polyclonal antibody (IgY fraction). |
Target Protein |
Human IL-8 |
Immunogen |
Highly purified recombinant human IL-8 |
Specificity |
This antibody recognizes human Interleukin-8 (IL-8) |
Purification |
Protein G affinity purified |
Species Reactivity |
Human, others not tested |
Cross Reactivity |
This polyclonal antibody exhibits no detectable cross-reactivity with human serum albumin, bovine serum albumin, and other cytokines tested such as IL-1beta, IL-16, TNF-alpha, GM-CSF, MCSF, bFGF and MIP-1alpha. |
Background |
Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis. |
Formulation |
Lyophilized from a solution in 0.01M PBS, pH 7.0 |
Reconstitution |
Double distilled water is recommended to adjust the final concentration to 1.00mg/mL |
Application info |
ELISA: The antibody when used at 2.6ng/mL can detect recombinant human IL-8 coated on ELISA plate.Neutralization: Total chicken IgY fraction at the concentration of 50 ug/mL showed 60% inhibition of 10ng/mL IL-8 induced IL8RB/293 cell migration Western Blot: The antibody, when used at concentration of 0.26 ug/mL will allow visualization of recombinant human IL-8. |
Research Area |
Cytokine, interleukin and chemotaxis |
Storage |
Store at -20C |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.