Item no. |
AE00357-1mg-B |
Manufacturer |
Aeonian Biotech
|
Amount |
1mg |
Category |
|
Type |
Antibody Monoclonal, recombinant |
Applications |
IHC |
Clone |
AE465R |
Specific against |
Human |
Host |
Rabbit |
Isotype |
IgG |
Formula |
1mg igG/ml in PBS with 0.05% azide upon quotation |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
carcinoembryonic antigen-related cell adhesion molecule 5; carcinoembryonic antigen; CD_antigen: CD66e; CEA cell adhesion molecule 5; meconium antigen 100; CEA; CD66e |
Available |
|
Description |
Specific, and tissue microarray-validated rabbit recombinant antibody clone AE465R to human CEACAM5 |
Principle name |
CEACAM5 |
immunogen |
Recombinant full-length human CEACAM5 protein |
purification method |
By Protein A from bioreactor concentrate |
Cell. Localization |
cell surface, plasma membrane |
Positive controls |
intestine |
RRID |
AB_2920702 |
IHC comments |
AE00357 to CEACAM5 was successfully used to stain epithelial plasma membranes in human colon, ileum and stomach sections. Recommended concentration: 1-3ug/ml |
Tissue Microarray Validation (Normal tissue) |
strong positive: appendix mucosa; colon mucosa; ectocervix; esophagus squamous epithelium; ileum mucosa; rectum mucosa; stomach; tonsilar surface epithelium; | weak to moderate: duodenum mucosa; sinus paranasalis; | no staining at all: adipose; adrenal gland; aorta, media; appendix muscular wall; bone marrow; breast; bronchus mucosa; cerebellum; cerebrum; colon muscular wall; duodenum Brunner gland; endocervix; endometrium; epididymis; fallopian tube; gallbladder; heart muscle; kidney; liver; lung; lymph node; myometrium; ovarian stroma; pancreas; parathyroid gland; parotid gland; pituitary gland; placenta; pregnant uterus; prostate; seminal vesicle; skeletal muscle; skin; spleen; testis; thymus ; thyroid gland; urinary bladder muscular wall; urothelium |
Tissue Microarray Validation (Cancer tissue) |
strong positive: adenocarcinoma of lung and pancreas; cholangiocarcinoma; colorectal adenocarcinoma; intestinal type gastric cancer; medullary thyroid cancer; ovarian mucinous carcinoma; squamous cell carcinoma in larinx and cervix | weak to moderate: diffuse gastric cancer; lobular breast cancer | no staining at all: hepatocellular carcinoma; malignant mesothelioma |
Expiration |
Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
Storage |
Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
Warranty |
This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
Liability |
This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.