Item no. |
AE00354-0.2mg-A |
Manufacturer |
Aeonian Biotech
|
Amount |
0.2mg |
Category |
|
Type |
Antibody Monoclonal, recombinant |
Applications |
IHC |
Clone |
AE022R |
Specific against |
Human |
Host |
Rabbit |
Isotype |
IgG |
Formula |
0.2 mg IgG/ml in PBS with 0.05% BSA & 0.05% azide. |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
B-cell receptor CD22; B-lymphocyte cell adhesion molecule; CD_antigen: CD22; Sialic acid-binding Ig-like lectin 2; T-cell surface antigen Leu-14; BL-CAM; SIGLEC-2; SIGLEC2 |
Available |
|
Description |
Specific, and tissue microarray-validated rabbit recombinant antibody clone AE022R to human CD22 |
Principle name |
CD22 |
epitope |
Extracellular domain between aa 52-178 |
immunogen |
Recombinant fragment corresponding to aa 52-178 of human CD22 protein |
purification method |
By Protein A from bioreactor concentrate |
Cell. Localization |
cell surface, plasma membrane |
Positive controls |
lymph node, spleen, tonsil |
RRID |
AB_2916358 |
IHC comments |
AE00354 to CD22 was successfully used to stain plasma membranes of B-cells in human lymph node, spleen, and tonsil sections. Recommended concentration: 1-3ug/ml |
Tissue Microarray Validation (Normal tissue) |
strong positive: appendix near lymph follicle; lymph node; spleen; tonsil | weak to moderate: gallbladder; ileum mucosa; sinus paranasalis; thymus | no staining at all: adipose; adrenal gland; aorta, media; appendix mucosa; appendix muscular wall; bone marrow; bronchus glands; bronchus mucosa; cerebellum; cerebrum; colon mucosa; colon muscular wall; duodenum Brunner gland; duodenum mucosa; ectocervix; endocervix; endometrium; epididymis; esophageal squamous epithelium; fallopian tube; heart muscle; kidney; liver; lung; myometrium; ovarian stroma; pancreas; parathyroid gland; parotid gland; pituitary gland; placenta; prostate; rectum mucosa; seminal vesicle; skeletal muscle; skin; stomach; testis; thyroid gland; urinary bladder muscular wall; urothelium |
Tissue Microarray Validation (Cancer tissue) |
strong positive: chronic lymphocytic leukemia in lymph node; diffuse large B-cell lymphoma; follicular B-cell lymphoma; mantle cell lymphoma; Warthin tumor | weak to moderate: Burkitt's lymphoma; Hodgkin's lymphoma; marginal zone lymphoma; high grade serous carcinoma; seminoma; vagina squamous cell carcinoma | no staining at all: Pheochromocytoma; colorectal adenocarcinoma; clear cell renal cell carcinoma; hepatocelular carcinoma; gastric adenocarcinoma; gastrointestinal stromal tumor; thyroid medullary carcinoma; urinary small cell carcinoma |
Expiration |
Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
Storage |
Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
Warranty |
This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
Liability |
This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.