Item no. |
AE00150-0.1mg-A |
Manufacturer |
Aeonian Biotech
|
Amount |
0.1mg |
Category |
|
Type |
Antibody Monoclonal |
Applications |
WB, IHC, ICC |
Clone |
EGP40 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG1 |
Formula |
200ug IgG/ml in PBS, 0.05% BSA, 0.05% azide |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial Cell Adhesion Molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal |
Available |
|
Description |
Selective and specific mouse monoclonal antibody clone EGP40/1372 to human EpCAM |
Principle name |
EpCAM |
epitope |
Extracellular domain (within aa 77-202 region) |
immunogen |
Recombinant fragment around aa 77-202 of human EpCAM protein (exact sequence is proprietary) |
purification method |
By Protein G from bioreactor concentrate |
Cell. Localization |
cell surface, plasma membrane |
Positive controls |
A431, carcinoma, HCT116, HEK293, lung, MCF7 |
RRID |
AB_2744511 |
IHC comments |
AE00150 to EpCAM was successfully used to stain cellular membranes in human colorectal and hepatocellular carcinoma sections. Recommended concentration: 0.3-1ug/ml |
WB comments |
AE00150 to EpCAM was successfully used to stain an approx. 40kDa band in human lung lysates and approx. 35kDa in lysates of cell lines HEK293, A431 and HCT116. Recommended concentration: 0.5-1.5ug/ml |
ICC comments |
AE00150 to EpCAM was successfully used to stain cellular membranes in cell line MCF7 and in human colorectal carcinoma. Recommended concentration: 1-3ug/ml |
Expiration |
Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
Storage |
Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
Warranty |
This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
Liability |
This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.