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GST Tag Antibody

GST Tag Antibody

Item no.	ABC-AW5668-U100
Manufacturer	Abcepta
Amount	100 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Glutathione S-transferase
Available
Antigen Type	Recombinant Protein
Application	WB
Bio Background	Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Bio References	Smith, D.B. and Johnson, K.S., (1988). Gene 67, 31. Parker, M.W. et al., (1990) J. Mol. Biol. 213, 221. Toye, B. et al., (1990) Infect. Immun. 58, 3909. Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262
Clonality	Monoclonal
Subtitle	Purified Mouse Monoclonal Antibody (Mab)
Legend image 1	All lanes : Anti-GST Tag Antibody at1:1000 dilution Lane 1: 293T/17 transfected with 12tag lysate(10ug) Lane 2: Non-transfected 293T/17 lysate(10ug) Lane 3: 293T/17 transfected with 12tag lysate(1ug) Lane 4: Non-transfected 293T/17 lysate(1ug) Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Anti-GST Tag Antibody at1:1000 dilution + 12tag recombinant protein Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Isotype	Mouse IgG1
Calculated MW	45-50KD
Antigen Region	NA
Antigen Source	HUMAN
Target/Specificity	Purified recombinant GST fusion protein was used to produced this monoclonal antibody.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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Delivery expected until 10/4/2024

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