Item no. |
ABC-AW5601-U100 |
Manufacturer |
Abcepta
|
Amount |
100 ul |
Category |
|
Type |
Antibody Primary |
Applications |
WB |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
ICAD, DFF45, DFFA, DFF-45, DFF1, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD, Inhibitor of CAD |
Available |
|
Primary Accession |
O00273 |
Antigen Type |
Synthesized Peptide |
Application |
WB |
Bio Background |
DFFA is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. |
Bio References |
Ninios, Y.P., et.al., Apoptosis 15 (2), 128-138 (2010)Banas, T., et.al., Eur. J. Obstet. Gynecol. Reprod. Biol. 146 (1), 87-91 (2009)Trynka, G., et.al., Gut 58 (8), 1078-1083 (2009) |
Clonality |
Polyclonal |
Gene ID |
1676 |
Gene Name |
DFFA |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-DFFA Antibody (C-term) at 1:2000 dilutionLane 1: Jurkat whole cell lysateLane 2: K562 whole cell lysateLane 3: Hela whole cell lysateLane 4: A431 whole cell lysateLane 5: A549 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:2000 |
Isotype |
Rabbit Ig |
Calculated MW |
36522 |
Antigen Region |
304-331 |
Antigen Source |
HUMAN |
Target/Specificity |
This DFFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-331 amino acids from the C-terminal region of human DFFA. |
NCBI Accession |
NP_004392.1; NP_998731.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.