Item no. |
ABC-AW5517-U100 |
Manufacturer |
Abcepta
|
Amount |
100 ul |
Category |
|
Type |
Antibody Primary |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
ACAT1, mitochondrial, MAT, ACAT, T2, Acetyl-CoA acetyltransferase, Acetoacetyl-CoA thiolase |
Available |
|
Primary Accession |
P24752 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. |
Bio References |
Locke, J.A., Prostate 68 (1), 20-33 (2008)Guo, Z.Y., Biochemistry 46 (35), 10063-10071 (2007)Haapalainen, A.M., Biochemistry 46 (14), 4305-4321 (2007) |
Clonality |
Polyclonal |
Gene ID |
38 |
Gene Name |
ACAT1 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilutionLane 1: HepG2 whole cell lysatesLane 2: mouse liver lysatesLane 3: SK-BR-3 whole cell lysatesLane 4: SW620 whole cell lysatesLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) (Cat.#AW5517), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:10~50 |
Isotype |
Rabbit Ig |
Calculated MW |
45200 |
Antigen Region |
311-349 |
Antigen Source |
HUMAN |
Target/Specificity |
This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-349 amino acids from the C-terminal region of human ACAT1. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.