Item no. |
ABC-AW5240-U100 |
Manufacturer |
Abcepta
|
Amount |
100 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
GPI; Glucose-6-phosphate isomerase; Autocrine motility factor; Neuroleukin; Phosphoglucose isomerase; Phosphohexose isomerase; Sperm antigen 36 |
Available |
|
Primary Accession |
P06744 |
Antigen Type |
Synthetic Peptide |
Application |
WB, FC |
Bio Background |
GPI belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. |
Bio References |
Shih, W.L., et al. Cancer Lett. 290(2):223-237(2010)Davila, S., et al. Genes Immun. 11(3):232-238(2010)Araki, K., et al. J. Biol. Chem. 284(47):32305-32311(2009)Tsutsumi, S., et al. Int. J. Oncol. 35(5):1117-1121(2009)Funasaka, T., et al. Cancer Res. 69(13):5349-5356(2009)Yanagawa, T., et al. J. Biol. Chem. 280(11):10419-10426(2005)Haga, A., et al. Biochim. Biophys. Acta 1480 (1-2), 235-244 (2000) |
Clonality |
Polyclonal |
Gene ID |
2821 |
Gene Name |
GPI {ECO:0000303|PubMed:2387591, ECO:0000312|HGNC:HGNC:4458} |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, Rat |
Legend image 1 |
Western blot analysis of lysates from rat liver tissue lysate, Ramos cell line (from left to right), using GPI Antibody (C-term)(Cat. #AW5240). AW5240 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
GPI Antibody (C-term) (Cat. #AW5240) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 2 |
FC |
Dilution image 2 |
1:10~50 |
Other Accession |
Q4R591 |
Predicted |
Pr |
Isotype |
Rabbit Ig |
Calculated MW |
63147 |
Antigen Region |
445-473 |
Antigen Source |
HUMAN |
Target/Specificity |
This GPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human GPI. |
NCBI Accession |
NP_000166.2; NP_001171651.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.