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ALDH2 Antibody

ALDH2 Antibody

Item no.	ABC-AW5219-U100
Manufacturer	Abcepta
Amount	100 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applications	WB, FC, IF, IHC-P
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	ALDH2; ALDM; Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI
Available
Primary Accession	P05091
Antigen Type	Recombinant Protein
Application	FC, IF, IHC-P, WB
Bio Background	This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix.
Bio References	Relationship between genetic polymorphisms of ALDH2 and ADH1B and esophageal cancer risk: a meta-analysis. Yang SJ, et al. World J Gastroenterol, 2010 Sep 7. PMID 20806441. Effects of alcohol-drinking behaviour and ADH1B and ALDH2 polymorphisms on basal DNA damage in human mononuclear cells as determined by the comet assay. Weng H, et al. Mutat Res, 2010 Aug 30. PMID 20685249. Evaluation of a brief web-based genetic feedback intervention for reducing alcohol-related health risks associated with ALDH2. Hendershot CS, et al. Ann Behav Med, 2010 Aug. PMID 20652463. Association of genetic polymorphisms of aldehyde dehydrogenase-2 with esophageal squamous cell dysplasia. Zhou YZ, et al. World J Gastroenterol, 2010 Jul 21. PMID 20632450. Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
Clonality	Monoclonal
Gene ID	217
Gene Name	ALDH2
Subtitle	Purified Mouse Monoclonal Antibody (Mab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of ALDH2 Antibody (Cat#AW5219) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of lysates from NCI-H292, A549, HepG2 cell line (from left to right), using ALDH2 Antibody(Cat. #AW5219). AW5219 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	ALDH2 Monoclonal Antibody (Cat. #AW5219) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	ALDH2 Monoclonal Antibody (Cat. #AW5219) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	NP_000681
Predicted	M, Rat
Isotype	IgG1
Calculated MW	56381
Antigen Region	262-537
Antigen Source	HUMAN
Target/Specificity	This ALDH2 Monoclonal antibody is generated from mouse immunized with ALDH2 recombinant protein.
NCBI Accession	NP_000681.2; NP_001191818.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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Delivery expected until 11/8/2024

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