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HIST1H2AG Antibody (Center)

HIST1H2AG Antibody (Center)

Item no.	ABC-AW5129-U100
Manufacturer	Abcepta
Amount	100 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	HIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p, H2A1
Available
Primary Accession	P0C0S8
Antigen Type	Synthetic Peptide
Application	IHC-P, FC, WB
Bio Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Bio References	Albig W., et al.Hum. Genet. 101:284-294(1997). Albig W., et al.Biol. Chem. 380:7-18(1999). Dobner T., et al.DNA Seq. 1:409-413(1991). Mannironi C., et al.DNA Cell Biol. 13:161-170(1994). Marzluff W.F., et al.Genomics 80:487-498(2002).
Clonality	Polyclonal
Gene ID	8329; 8330; 8332; 8336; 8969
Gene Name	H2AC11 (HGNC:4737)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Western blot analysis of lysates from mouse NIH/3T3, CEM, Hela, HepG2 cell line (from left to right), using HIST1H2AG Antibody (Center)(Cat. #AW5129). AW5129 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Immunohistochemical analysis of paraffin-embedded M. stomach section using HIST1H2AG Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 2	IHC-P
Dilution image 2	1:25
Legend image 3	Immunohistochemical analysis of paraffin-embedded R. stomach section using HIST1H2AG Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 3	IHC-P
Dilution image 3	1:25
Legend image 4	Immunohistochemical analysis of paraffin-embedded M. testis section using H. stomach Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 4	IHC-P
Dilution image 4	1:25
Legend image 5	Immunohistochemical analysis of paraffin-embedded M. pancreas section using HIST1H2AG Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 5	IHC-P
Dilution image 5	1:25
Legend image 6	Flow cytometric analysis of Hela cells using HIST1H2AG Antibody (Center)(green, Cat#AW5129) compared to an isotype control of rabbit IgG(blue). AW5129 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Type image 6	FC
Dilution image 6	1:25
Other Accession	P84051; P27661; P16104; Q7ZUY3; A9UMV8; Q8R1M2; Q4R3X5; Q9BTM1; P70082; Q3ZBX9; Q00728; P02263; Q4FZT6; Q8BFU2; Q7L7L0; P35062; P04912; Q64523; Q16777; A1A4R1; Q64522; Q8IUE6; P0CC09; Q6GSS7; Q6FI13; P04911; P06897; P02262; P22752; P0C0S9; Q8CGP7; Q99878;
Predicted	Rat, B, X, Y, C, Pr, Zf, D
Isotype	Rabbit Ig
Calculated MW	14091
Antigen Region	63-87
Antigen Source	HUMAN
Target/Specificity	This HIST1H2AG antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 63-87 amino acids from the Central region of human HIST1H2AG.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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Delivery expected until 9/6/2024

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